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N8540-05-L Nutrient Mixture Ham’s F-10 w/HEPES, w/o L-Glutamine (Liquid) CAS:

Specifications
References
Grade
Cell Culture Grade
Shipping Temp
Blue Ice
Storage Temp
4°C

Ham’s Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Ham’s F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.

pH
As Reported
Endotoxin
≤1EU/ml
Storage and Stability
Store at 4°C. Stable for 6 months after receipt.
References
1. Ham, R.G., Exp. Cell Res. 29: 515-526. (1963). 2. Ham, R.G., Proc. Nat. Acad. Sci. 53: 288-293. (1965).
USBio References
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