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P2488-05 PAK 1 PBD (Rac/cdc42) Assay Reagent, Recombinant, Human (Agarose) CAS:

Specifications
References
Grade
Purified
Conjugate
Agarose
Swiss Prot
P60953
Accession Number
NM_001039802.1,|NM_001791.3,|NM_044472.2
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

GST-tagged fusion protein to residues 67-150 of human PAK-1, expressed in E. coli, bound non-covalently to glutathione-agarose.

Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates. Species Crossreactivity: Predicted to interact with all mammalian Rac and cdc42.
Applications
Suitable for use in Affinity Precipitation and Immmunoblot. Other applications have not been tested.
Recommended Dilutions
Affinity Precipitation: 10ug precipitated Rec-GTP and cdc42-GTP fromn HeLa cell lysates that were loaded in vitro with GTP-gammaS and GDP. Immunoblot: 1ug/ml Optimal dilution to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE and Coomassie blue staining)
Concentration
As Reported
Form
Supplied as a liquid suspension, bound to glutathione agarose beads; provided as a 50% slurry in PBS, pH 7.4, 50% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Benard, V., et al., JBC 274: 13,198–13,204 (1999). 2. Chang, S.H., et al., Nature 410: 944-948 (2001). 3. Taylor, S.J. and Shalloway, D., Current Biology 6: 1621–1627 (1996).
USBio References
No references available
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