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P3291-96M PGLS, Recombinant, Human (6PGL, 6-phosphogluconolactonase) CAS:

Specifications
References
Grade
Highly Purified
Accession Number
NP_036220
Molecular Weight
29.7
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

6-phosphogluconolactonase, also known PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate.

Source
Recombinant corresponding to aa1-258 of human PGLS protein, fused to His-tag at N-terminus, expressed in E.coli.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL
Molecular Weight: 29.7kD (278aa) confirmed by MALDI-TOF
Enzyme Activity
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~95% (SDS-PAGE).
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol, 100mM sodium chloride.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Collard F., et al. (1999) FEBS Lett. 459(2):223-6. 2. Hong M K., et al. (2008) Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(11):1016-8.
USBio References
No references available
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