USBio Logo

S1013-61B Siglec 8, CT (Sialic Acid-binding Ig-like Lectin 8, Siglec 8, Sialoadhesin Family Member 2, SAF-2, CDw329, CD329, SAF2) CAS:

Specifications
References
Grade
Purified
Swiss Prot
Q9NYZ4
Specificity
Recognizes human SIGLEC8. Species Crossreactivity: mouse.
Applications
E IHC WB
Molecular Weight
54.042
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C

SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
References
1. Foussias, G., et al., Biochem. Biophys. Res. Commun. 278(3):775-781 (2000). 2. Floyd, H., et al., J. Biol. Chem. 275(2):861-866 (2000). 3. Kikly, K.K., et al., J. Allergy Clin. Immunol. 105(6 Pt 1), 1093-1100 (2000).
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved