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S7971-25 STAT3, Control Peptide (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) CAS:

Specifications
References
Grade
Highly Purified
Molecular Weight
1711
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Control peptide for the STAT 3 rabbit polyclonal antibody, S7971-20.

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1), and is required for murine fetal development (2). Additionally, Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 via the MAPK or mTOR pathway (8,9). Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3a (86 kD) and Stat3b (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3b lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
Sequence (linear): H2N-Ser-Leu-Met-Gln-Asn-Asn-Gly-Glu-Gly-Ala-Glu-Pro-Ser-Ala-Cys-NH2
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
≥90% by HPLC analysis (purity based on peak area)
Concentration
~0.5mg net peptide/vial
Form
Supplied as a lyophilized powder (Trifluoroacetate salt).
Important Note
This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
References
(1) Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75–120. (2) Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801–227. (3) Catlett-Falcone, R. et al. (1999) Immunity 10, 105–115. (4) Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79–85. (5) Bromberg, J.F. et al. (1999) Cell 98, 295–303. (6) Darnell Jr., J.E. et al. (1994) Science 264, 1415–1421. (7) Ihle, J.N. (1995) Nature 377, 591–594. (8) Wen, Z. et al. (1995) Cell 82, 241–250.|General References:|1. Spieker-Polet H, et al. Proc Natl Acad Sci. 1995 Sep 26;92(20):9348-52. 2. Liguori MJ, et al. Hybridoma. 2001 Jun; 20(3):189-98. 3. G.Cano1, F. Milanezi2, D. Leitao2,3, S. Ricardo2, M.J. Brito1, F. C. Schmitt2-3 1Garcia da Orta Hospital, Almada, Portugal,2 Inst. Molec. Pathology and Immunology of Porto University, Portugal,3 Medical Faculty of Porto university, Portugal Diagn Cytopathol, 2003 Oct; 29(4): 207 -11. 4. L.K. Diaz* and N.Sneige *Department of Pathology,Northwestern University, Chicago,+ Department of Pathology, University of Texas, Huston, Adv Anat Pathol,2005; 12(1), 10-19. 5. Z. Huang1, W. Zhu2, G. Szekeres3, H. Xia1 1Spring Bioscience Corp, Fremont,CA, 2 Epitomics Inc, Burlingame,CA, , 4Histopathology Ltd, Hungary, Appl Immunohistochem Mol Morphol. 2005; 13 (1): 91-95 6. S. Rossi1, E. Orvieto1, S.Chinellato1, A. Furlanetto1, L.Laurino1, F. Facchetti2, AP Dei Tos 2 1Department of Pathology, 2Treviso, Italy; *Brescia, University School of Medicine, Brescia, Italy., Abstract presented at USCAP 2004. Modern Pathology 2004; 17 (suppl 1): 361A 7. M. Blechner, E. Ballesteros, D. Mandich, D. Stevens, R. Cartun, Hartford Hospital, Hartford, CT. Abstract presented at USCAP 2004. Modern Pathology 2004; 17 (suppl 1): 241A 8. W. Cheuk, K.O.Y. Wong, C.S.C. Wong and J.K.C. Chan, Department of Pathology, Queen Elizabeth Hospital, Hong Kong, Am J Surg Path, 2004; 28 (6): 801-807. 9. G.B. Budd, E. Tso, B. Yoder, T. Choueiri, P. Elson, S. Tarr, M. Skacel, R. Tubbs, A. Dawson, D. Hicks, Cleveland Clinic Foundation, Cleveland, OH, Abstract presented at ASCO Annual meeting, June 2004, New Orleans 10. S. M. Tarr, S. Short, K. Hansen, T. Morken, H. Xia, E. Downs-Kelly, R. R. Tubbs, D. G. Hicks, Department of Pathology and Laboratory Medicine. The Cleveland Clinic Foundation, Cleveland, Ohio. Lab Vision Corp., Fremont, Ca., Spring Bioscience Corp, Fremont ,CA, Abstract presented at Association for Molecular Pathology meeting, Los Angeles, 2004 11. A.M. Gown, T.S. Barry, P. Kandalaft, L.C. Goldstein, C.C. Tse and D.O. Treaba, Clinical Research Division , PhenoPath Laboratories and IMPRIS, Seattle, WA, Abstract presented at USCAP 2005. Modern Pathology 2005; 18, suppl.1,pag 35A 12. D.O. Treaba, A.W. Hing, L.C. Goldstein, T.S. Barry, P. Kandalaft, C.B. Gilks, T.O. Nielsen and A.M. Gown, Clinical Research Division , PhenoPath Laboratories and IMPRIS, Seattle, WA, USA Genetic Pathology Evaluation Centre, University of British Columbia, Vancouver, BC, Canada, Abstract presented at USCAP 2005. Modern Pathology 2005; 18, suppl.1,pag 53A 13. S. Rossi1, L. Laurino1, A. Furlanetto1, S.Chinellato1, E. Orvieto1, F. Canal1, F. Facchetti2, A.P. Dei Tos1 1 Depart. Pathology, Hospital of Treviso, Italy, 2 Brescia University School of Medicine, Brescia, Italy, Am J Clin Pathol, 2005, Aug;124(2):295-302
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