Recognizes rat Thiazide-Sensitive Na-Cl Transporter (TSC, NCC). The immunogen shows no significant sequence homology with other CCC. Species sequence homology: mouse and rabbit: 98% and human 83%.
Applications
E
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Control Peptide for T9100-10 (antiserum) and T9100 (affinity purified)
Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+-Cl-cotransporters (NCC or TSC.
Applications
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution
Antibody Blocking: 5-10ug per 1ul T9100-10 (antiserum) or per 1ug T9100-10A (affinity purified antibody). ELISA: 1ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Rat synthetic peptide
Purity
Highly purified
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, pH 7.5, 0.1% sodium azide, 40% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
(1). Gamba G et al (1994) J Biol. Chem. 269, 17713-17722; Simon DB et al (1996) Nature Genet. 12, 24-30; Mount DB et al (1995) J Am. Soc. Nephrol. 6, 347; Igarashi P et al (1995) Am. J. Physiol. 269, F405-418; Haas M et al (1987) Am. J. Physiol. 253, C243; Mount DB et al (1998) J. Exptl. Biol. 201, 2091 (review).