Cholesterol acyltransferase 1 is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer's disease.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Western Blot: 0.05ug/ml Immunohistochemistry: 1:1000 Optimal dilutions to be determined by the researcher.

Caco-2 cell lysate

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic linear peptide corresponding to human Cholesterol acyltransferase 1 (KLH).

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purified by immunoaffinity chromatography.

Recognizes human Cholesterol Acyltransferase 1. Species sequence homology: Rhesus macaque, chimpanzee, rat, mouse (93%).