CRP

Clone Type

Monoclonal

Host

Mouse

Source

E. coli

Isotype

IgG1

Clone Number

1D8D9

Grade

Affinity Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Notes

Preservative Free

CRP (also known as cAMP receptor protein) is a regulatory protein in bacteria. CRP binding to cAMP leads to conformational changes in CRP resulting in a dramatic increase in its binding to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA and acts as a negative regulator of its own synthesis.

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 0.25-2ug/ml Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Purified protein

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Purity

Purified by affinity chromatography.

Specificity

Recognizes E. coli CRP.

References

1. Lauritsen I, et al. 2021. Nat Commun. 12:5880. |2. Ayhan D, et al. 2016. PLoS Biol. 14: 1002552. |3. Pryor R et al. 2019. Cell. 178(6):1299-1312 . |4. Seok JY, et al. 2021. Cell Reports. 36(8):109589.