CD54

Clone Type

Monoclonal

Host

Mouse

Source

Human

Swiss Prot

P05362

Isotype

IgG1,k

Clone Number

15.2

Grade

Purified

Applications

E FC IC IP WB

Crossreactivity

Hu Po

Shipping Temp

Blue Ice

Storage Temp

-20°C

Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes.

Applications

Suitable for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Rheumatoid synovial cells and human monocytes

Form

Supplied as a liquid in BBS, pH 8.2.

Purity

Purified

Specificity

Recognizes human CD54. Species Crossreactivity: porcine

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137767 Mouse Anti-CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58)

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