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227415-ML490 Mouse Anti-CD34 (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Canine
Swiss Prot
Q28270
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
1H6
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Ca
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Specific for canine homologue of CD34, a glycosylated type 1 transmembrane protein of ~110kD (McSweeney et al. 1998) expressed on the cell suface of endothelial cells and haematopoietic stem cells.
Key marker of canine hematopoietic progenitor cells.
Reported for use in CD34+ enrichment studies, (Goerner, M. et al. 2001) and (Horn, P. et al. 2004).
Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Canine CD34 fusion protein.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes canine CD34.
References
1. McSweeney, P. et al. (1998) Characterization of monoclonal antibodies that recognize canine CD34. Blood. 91: 1977-1986. 2. Goerner, M. et al. (1999) The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene tranfer into hematopoietic repopulating cells in dogs. Blood. 94: 2287-2292. 3. Bhattacharya, V. et al. (2000) Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34+ bone marrow cells. Blood. 95: 581-585. 4. Goerner, M. et al. (2001) Sustained multilineage gene persistence and expression in dogs transplanted with CD34+ marrow cells transduced by RD114-pseudotype oncoretrovirus vectors. Blood. 98: 2065-2070. 5. Horn, P. et al. (2004) Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol. Blood. 103: 3710-3716. 6. Avallone, G. et al. (2007) The spectrum of canine cutaneous perivascular wall tumors: morphologic, phenotypic and clinical characterization. Vet Pathol. 44(5):607-20. 7. Palmieri, C. et al. (2012) Use of Electron Microscopy to Classify Canine Perivascular Wall Tumors Veterinary Pathology August 3 [epub ahead of print] 8. Georges, G. et al. (2001) Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes. Blood. 98:3447-55.
USBio References
No references available
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