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B2702-01D-FITC Mouse Anti-Brain Natriuretic Peptide, Pro, NT, aa 13-27 (NT-proBNP, N-terminal prohormone brain natriuretic peptide) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P16860
Conjugate
FITC
Isotype
IgG1
Clone Number
10B302 (18H5)
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

NT-proBNP is a cleavage product during the proteolytic activation of BNP. NT-proBNP is a secreted protein which is a member of the natriuretic peptide family, which is predominantly secreted from the cardiac ventricles in response to volume and pressure overload. NT-proBNP is more stable than BNP in serum, and has become a useful biomarker of heart failure.

Applications
Suitable for use in FLISA and Western Blot. Other applications have not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
aa13-27 from N-terminal of Pro Brain Natriuretic Peptide
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the amino-terminal fragment at aa13-20 of human pro-brain natriuretic peptide at ~9kD.
References
1. Clerico, A. et al. (2006) Clinical relevance of biological variation: the lesson of brain natriuretic peptide (BNP) and NT-proBNP assay. Clin. Chem. Lab. Med. 44: 366-378, 2. Pfister, R. and Schneider, C.A. (2004) Natriuretic peptides BNP and NT-pro-BNP: established laboratory markers in clinical practice or just perspectives? Clin. Chim. Acta. 349: 25-38.
USBio References
No references available
Conjugates
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