Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Flow Cytometry. Other applications not tested.

Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Western Blot: Detetcts a band at ~95kDa in Raji cell lysates under reducing conditions Optimal dilutions to be determined by the researcher.

I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Positive Control: Tonsil

I1904-78R: IgG1 Murine Negative Control

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months aafter receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Peptide corresponding to human CD19, CGPDPAWGGGGRMGTWSTR (C-terminal), conjugated to KLH.

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes the C-terminal cytoplasmic tail sequence, CGPDPAWGGGGRMGTWSTR of human CD19.