Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Immunohistochemistry: frozen Optimal dilution determined by the researcher.

Goat anti mouse IgG:HRP (rat adsorbed) Goat anti mouse IgG:RPE (rat adsorbed)

I1904-78Y IgG1, Murine Negative Control (For rat tissue)

Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

PHA stimulated rat lymphocytes

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purified by Protein G affinity chromatography.

Recognizes rat CD45C., the high molecular weight form of the leucocyte common antigen found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells but splits CD4+ve T cells into two populations differing in their helper activities.