CD53

Clone Type

Monoclonal

Host

Mouse

Source

Human

Swiss Prot

P19397

Isotype

IgG1

Clone Number

3H1544 (MEM-53)

Grade

Affinity Purified

Applications

FC IHC IP WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD53 cell surface antigen is also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.

Applications

Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions

Western Blot: Non-reducing conditions Flow Cytometry: 1:10-1:25. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes the human CD53 cell surface antigen.

References

1. Bazil, V. et al. (1989) Folia Biol (Praha). 35 (5): 289-97. 2. Angelisová P et al. (1990). Immunogenetics. 32 (4): 281-5. 3. Mollinedo, F. et al. (1997) Clin Diagn Lab Immunol. 4: 229-31. 4. de Winde, C.M. et al. (2015) Histochem Cell Biol. 144 (2): 133-46. 5. Matsumoto K et al. (1999) Int Arch Allergy Immunol. 120 Suppl 1: 38-44. 6. Spring, F.A. et al. (2013) . PLoS One. 8 (5): e62654. 7. Tippett, E. et al. (2013) . J Leukoc Biol. 93 (6): 913-20. 8. Zuidscherwoude, M. et al. (2015) . Sci Rep. 5: 12201.|

USBio References

No references available

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C2408-11A Mouse Anti-CD53

Clone Type

Host

Source

Swiss Prot

Isotype

Clone Number

Grade

Applications

Crossreactivity

Shipping Temp

Storage Temp

References

USBio References

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