Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 1:5-1:10, 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human thymic clone B12

Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Purified by Protein G affinity chromatography from tissue culture supernatant

Recognizes human CD101. Reported to inhibit allogeneic T-cell responses (ref. 1)