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C2448-04J-ML650 Rabbit Anti-CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (MaxLight 650)

Specifications
Brand
MaxLight™
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
MaxLight™650
Isotype
IgG
Grade
Purified
Applications
IP WB
Crossreactivity
Hu
Accession #
NP_000213.1, BC071593
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

The c-kit proto-oncogene is the cellular homologue of the feline sarcoma v-kit oncogene. It encodes a type III transmembrane receptor tyrosine kinase also known as CD117 or stem cell factor receptor (SCFR). c-Kit signaling is activated when a ligand known as steel factor or stem cell factor (SCF) binds to the receptor. Interactions between specific phosphotyrosine residues of activated receptor tyrosine kinases (RTKs) and Src homology 2 (SH2) domains define cellular growth and differentiation signals. It has been reported that SCF stimulates the association of the p85 subunit of PI3K with the phosphorylated tyrosine-721 residue within wild type kit. Subsequently, PI3K contributes to the activation of Akt and JNKs. c-Kit is necessary for the development of hematopoietic progenitors, mast cells, germ cells, melanocytes, and the interstitial cells of Cajal (ICC). Oncogenic c-kit mutations have been implicated in neoplasms arising from these stem cell lineages, such as gastrointestinal stromal tumor (GIST). The mature, phosphorylated form of c-Kit is a glycosylated protein with a molecular weight of ~145kD, while the immature form is ~125kD.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Western Blot: 1-3ug/ml Immunohistochemistry: 5-10ug/ml Note: Prior to immunohistochemistry with paraffin-embedded tissue sections, heat induced epitope retrieval with EDTA, pH 8.0. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from an internal sequence of the human c-Kit protein
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by epitope affinity chromatography.
Specificity
Reacts with the human c-Kit (CD117 or stem cell factor receptor (SCFR)) oncoprotein. On Western blots, this antibody identifies a ~145kD band representing the mature (phosphorylated, glycosylated) form of c-Kit, a ~125kD band for the immature form, and a ~95kD band representing the soluble form. Reactivity has been confirmed with human GIST-882 and EWS-502 cell lysates.
Conjugates
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