DIO2, ID

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

Q92813

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Accession #

NP_000784.2

Shipping Temp

Blue Ice

Storage Temp

-20°C

DIO2 belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide selected from the Center region of human DIO2 (KLH).

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human DIO2.

References

1. He,B.,et.al., Prog. Neuropsychopharmacol. Biol. Psychiatry 33(6), 986-990 (2009). 2. Heemstra,K.A., et.al., J. Clin. Endocrinol. Metab. 94(6), 2144-2150 (2009).