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D9807-19 Anti-Dulbecco’s MEM (DMEM) F-12 w/o Choline Chloride, Phenol Red

Specifications
References
Grade
Cell Culture Grade
Shipping Temp
RT
Storage Temp
RT/4°C

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance
White to off-white, homogenous, free flowing powder
Solubility
Colorless to light yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
References
1. Gibb AA, et al. Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes. Biochem J. 2017 Jul 13. pii: BCJ20170474. doi: 10.1042/BCJ20170474. 2. Biesemann N., et al. High throughput screening of mitochondrial bioenergetics in human differentiated myotubes identifies novel enhancers of muscle performance in aged mice. 2018. Sci Rep. 8(1):9408. doi: 10.1038/s41598-018-27614-8.
USBio References
1. Gibb AA, et al. Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes. Biochem J. 2017 Jul 13. pii: BCJ20170474. doi: 10.1042/BCJ20170474. 2. Biesemann N., et al. High throughput screening of mitochondrial bioenergetics in human differentiated myotubes identifies novel enhancers of muscle performance in aged mice. 2018. Sci Rep. 8(1):9408. doi: 10.1038/s41598-018-27614-8.
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