Green Fluorescent Protein

Clone Type

Polyclonal

Host

Goat

Swiss Prot

P42212

Isotype

IgG

Grade

Affinity Purified

Applications

E IF WB

Shipping Temp

Blue Ice

Storage Temp

-20°C

Green fluorescent protein (GFP) is a 27kD protein which is derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression.

Applications

Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions

ELISA: 1:10,000-1:30,000 Western Blot: 1:400-1:10,000 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Fusion protein corresponding to full length GFP derived from Aequorea Victoria.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by affinity chromatography.

Specificity

Recognizes wild type GFP, but will also detect recombinant and enhanced forms of GFP.

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G8965-01P Goat Anti-Green Fluorescent Protein (GFP)

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