Insulinlike Growth Factor II, a 67aa mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen. IGFII is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulinlike growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGFII is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (2).

Suitable for use in FLISA, Functional Assays, and Western Blot. Other applications not tested.

FLISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 3.1-4.6ug/ml Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Recombinant human IGF-II.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by immunoaffinity chromatography.

Recognizes human IGF-II.