Keratin 10 (CK 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.

Applications: Immunofluorescence, Western Blotting, Immunohistochemistry.

Western Blotting: 1ug/ml Immunohistochemistry: 2-4ug/ml. Frozen & formalin/paraffin. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Optimal dilutions to be determined by the researcher.

Note: Applications are based on unconjugated antibody.

Cytoskeletal preparation extracted from human ectocervical epithelium. Cellular Localization: Cytoplasmic.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography.

Recognizes human Keratin 10 at 56.5kD. Species Crossreactivity: rat, canine and feline.