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M2414-ML650 Mouse Anti-Mast Cell Chymase (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P23946
Conjugate
MaxLight™650
Isotype
IgG1,k
Clone Number
2Q1505 (B7)
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Accession #
NP_001827
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Immunohistochemistry. Not suitable for use in formaldehyde fixed tissue. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): Heat induced epitope retrieval with citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified Mast Cell Chymase from human skin.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human mast cell chymase (27kD).
References
1. Tadashi, T., et al., J. Hepatology 33: 961-966 (2000). 2. Buckley, M., et al., J. Pathol. 186: 67-74 (1998). 3. Kleinjan, A., et al., Allergy 51: 614-620 (1998). 4. Clin. Exp. Allergy 20: 53-58 (1990). 5. J. Allergy Clin. Immunol. 86: 34-39 (1990). 6. J. Immunol. 145: 2652-2661 (1990). 7. Lab. Invest. 63: 581-585 (1990). 8. Lab. Invest. 58: 682-691 (1989). 9. Lab. Invest. 60: 147-157 (1989). 10. J. Histochem. Cytochem. 37: 1509-1515 (1989). 11. PNAS USA 86: 10,039-10,043 (1989). 12. J. Immunol. 138: 2184-2189 (1987). 13. J. Immunol. 138: 4381-4386 (1987). 14. J. Immunol. 138: 2611-2615 (1987). 15. PNAS USA 83: 4464-4458 (1986).
USBio References
No references available
Conjugates
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