MCM-5 (mini chromosome maintenance protein 5), a protein with an important role in the control of DNA replication. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.

Tonsil/Cervical Carcinoma

Spleen cells from immunized CBI/CFI mice were fused with cells of the mouse SP2 myeloma cell line.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Bacterially expressed human Mcm5.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes human MCM-5.