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O5202-ML550 Mouse Anti-Occludin (MaxLight 550)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™550
Isotype
IgG1,k
Clone Number
3G217
Grade
Affinity Purified
Applications
FLISA IC IF WB
Crossreactivity
Ca Hu Ma Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).

Applications
Suitable for use in FLISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Control
T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma).
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
GST fusion protein consisting of the C-terminal region (~150aa) of human occludin. Species Sequence Homology: Bacteria, C.elegans, fish, guinea pig
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Occludin Protein. Species Crossreactivity: mouse, rat and canine. Does not react with sheep, porcine, rabbit, rhesus monkey
References
1. Anderson, J.M., et al., Curr. Opin. Cell. Biol. 5: 772-778 (1993). 2. Gumbiner, B., Am. J. Physiol. 123: 1631-1633 (1993). 3. Farquhar, M., and Palade, G., J. Cell. Biol. 17: 375-409 (1963). 4. Stevenson, B.R., et al., J. Cell. Biol. 103: 755-766 (1986). 5. Anderson, J.M. et al. (1988) J. Cell. Biol. 106:141-1149. 6. Itoh, M., et al., J. Cell. Biol. 115: 1449-1462 (1993). 7. Gumbiner, B., et al., PNAS USA 88: 3460-3464 (1991). 8. Citi, S., et al., Nature 33: 272-276 (1988). 9. Zhong, Y., et al., J. Cell. Biol. 120: 477-483 (1993). 10. Keno, B.H., et al., J. Cell. Biol. 134: 1003-1018 (1996). 11. Furuse, M., et al., J. Cell Biol. 123: 1777-1788 (1993). 12. Furuse, M., et al., J. Cell. Biol. 127: 1617-1626 (1994). 13. Ando-Akatsuka, Y., et al., J. Cell. Biol. 133: 43-47 (1996).
USBio References
No references available
Conjugates
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