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S1013-60P-Biotin Mouse Anti-Siglec 7 (Sialic Acid-binding Immunoglobulin-like Lectin, CDw328) (Biotin)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q9Y286
Conjugate
Biotin
Isotype
IgG1
Clone Number
9A202 (5-386)
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Siglec 7 is a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. Siglec 7 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:100-1:200; 10ul labels 1x10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:100 Optimal dilutions to be determined by the researcher.
IHC Positive Control Tissue
Frozen tonsil
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Monocyte-derived dendritic cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human Siglec 7.
References
1. Falco, M. et al. (1999) Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells. J. Exp. Med. 190: 793-801. 2. Nicoll, G. et al. (1999) Identification and characterization of a novel Siglec, Siglec-7, expressed by human natural killer cells and monocytes*. J. Biol. Chem. 274: 34089-34095.
USBio References
No references available
Conjugates
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