SIRP alpha is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Flow Cytometry: 1:100-1:200. 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:100 Optimal dilutions to be determined by the researcher.

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

I1904-83P1: IgG2a Murine Negative Control

Positive Control: Human tonsil

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Monocyte-derived dendritic cells.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes human SIRP alpha.