Suitable for use in Immunofluorescence, FLISA and Western Blot. Other applications not tested.

FLISA: 0.1-1ug/ml Western Blot: 0.5-1ug/ml Immunofluorescence: 5-10ug Optimal dilutions to be determined by the researcher.

HeLa cell extracts and HeLa cell nuclear extracts

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

A synthetic peptide corresponding to aa84-104 of the human SRp20 protein

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).

Purified by Protein A affinity chromatography.

Recognizes human non-snRNP splicing factor SRp20 and does not crossreact with other SR protein family members. Reactivity is not dependent upon phosphorylation of the SRp20 protein.