IgG2b CAS

Brand

MaxLight™

Grade

Affinity Purified

EU Commodity Code

30021010

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Notes

Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications

Suitable for use in FLISA. Other applications not tested.

Recommended Dilutions

FLISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher.

Avidity for rat IgG2b: 4.7 x 10e9 M-1.

Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage and Stability

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

References

1. Sato, K., et al., Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat transplant. J. Immunol. 166: 4185-4194 (2001).