The HSP90beta (C-Terminal) Inhibitor Screening BioAssay™ Kit is designed to measure the inhibition of HSP90beta binding to its protein target PPID (also known as Cyclophilin D). The assay kit comes in a convenient AlphaLISA® format, with enough HSP90beta (527-724), assay buffer, detection buffer, and purified GST-tagged PPID to perform a total of 384 enzyme reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing HSP90beta, PPID, and an inhibitor of choice is incubated for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.