RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
| Components | mg/L |
| Calcium Nitrate•4H2O | 100 |
| Magnesium Sulfate | 48.84 |
| Potassium Chloride | 400 |
| Sodium Bicarbonate | 1500 |
| Sodium Chloride | 6000 |
| Sodium Phosphate Dibasic | 800 |
| L-Arginine | 200 |
| L-Asparagine | 50 |
| L-Aspartic Acid | 20 |
| L-Cystine•2HCl | 65 |
| L-Glutamic Acid | 20 |
| L-Glutamine | 300 |
| Glycine | 10 |
| L-Histidine | 15 |
| L-Hydroxyproline | 20 |
| L-Isoleucine | 50 |
| L-Leucine | 50 |
| L-Lysine•HCl | 40 |
| L-Methionine | 15 |
| L-Phenylalanine | 15 |
| L-Proline | 20 |
| L-Serine | 30 |
| L-Threonine | 20 |
| L-Tryptophan | 5 |
| L-Tyrosine•2Na•2H2O | 29 |
| L-Valine | 20 |
| p-Aminobenzoic Acid | 1 |
| Biotin | 0.2 |
| Choline Chloride | 3 |
| Vitamin B12 | 0.005 |
| Folic Acid | 1 |
| i-Inositol | 35 |
| Niacinamide | 1 |
| D-Pantothenate•Ca | 0.25 |
| Pyridoxine•HCl | 1 |
| Riboflavin | 0.2 |
| Thiamine•HCl | 1 |
| D-Glucose | 4500 |
| Glutathione (reduced) | 1 |
| HEPES | 2383 |
| Phenol Red | 5 |
| Sodium Pyruvate | 110 |
| Total: | 16.9g/ml |
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