USBio Logo

T2810-10 Terrific Broth, Modified for Genomics (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Potassium phosphate dibasic, Dextrose, Tryptone, Yeast Extract, Soytone

Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates. T2810-10 is useful for obtaining the highest yields of plasmid DNA from E.coli by the addition of a proprietary blend of vitamins, micronutrients and carbohydrates. Will produce up to 300% greater growth than traditional Terrific Broth!. Doubling time will actually decrease from 60 to 20 minutes, thus giving the same cell yield as Terrific Broth in 1/3 the time.

BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.
Homogeneous: Proprietary blending technology ensures complete mixing of components.
Appearance
Light tan, homogeneous, free flowing powder
Solubility
Light yellow, clear, complete
pH
7.3 ± 0.2
Directions per Liter
Dissolve 35 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). If precipitate forms on autoclaving, medium may be filter-sterilized using a 0.2 micron filter.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Media Formulation
Components shown as g/liter
Dextrose2.5
Potassium Phosphate Dibasic2.5
Sodium Chloride5.0
Soytone3.0
Tryptone17.0
Yeast Extract5.0
References
1. Tartof and Hobbs (1987).
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved