Tryptose, Sodium Chloride, Sodium Phosphate, Dibasic, Glucose

Tryptose Phosphate Broth is a buffered dextrose broth for use as an adjuvant to tissue culture media and for the cultivation of fastidious bacteria. Ginsberg, et al., maintained tissue cultures of HeLa cells for at least 10 days in a mixture of 15-25% Tryptose Phosphate Broth, 67.5-77.5% Scheren maintenance solution and 7.5% chicken serum. The cells increased 3-5 fold in number during this period. Smaller quantities of ARD, AD and type 1 poliomyelitis virus could be detected and more ARD virus could be propagated in HeLa cells in the Tryptose Phosphate. Tryptose Phosphate Broth is recommended for the cultivation of streptococci, pneumococci, meningococci and other fastidious organisms. Tryptose Phosphate Broth with added agar and sodium azide is recommended for the isolation of pathogenic streptococci from cheese and other dairy products (2,3). Tryptose Phosphate Broth with added agar is also recommended by the American Public Health Association for the examination of throat swabs and blood for Streptococcus pneumoniae and as a growth medium for pneumococci prior to the bile solubility test.