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029700 SNRPD3, Recombinant, Human, aa1-126, His-Tag (Small Nuclear Ribonucleoprotein Sm D3, Sm-D3, snRNP Core Protein D3) CAS:

Specifications
References
Grade
Purified
Accession Number
NP_004166
Molecular Weight
16
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that comprises the spliceosome in eukaryotes. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Recombinant corresponding to aa1-126 from human SNRPD3, fused to His-tag at N-terminal expressed in E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNKN QGSGAGRGKA AILKAQVAAR GRGRGMGRGN IFQKRR
Molecular Weight
~16kD (MALDI-TOF)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥85% (SDS-PAGE)
Concentration
~0.1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 40% glycerol, 0.5M sodium chloride, 2mM DTT, 0.1mM PMSF.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Lehmeier T. et al. (1995) Proc Natl Acad Sci. 91:12317.
USBio References
No references available
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